Figure 2. IHC analysis of SOD2/Mnsod using anti-SOD2/Mnsod antibody (PA1776). SOD2/Mnsod was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-SOD2/Mnsod Antibody (PA1776) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of SOD2/Mnsod using anti-SOD2/Mnsod antibody (PA1776). SOD2/Mnsod was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-SOD2/Mnsod Antibody (PA1776) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-SOD2 Antibody
PA1776
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetSOD2
Overview
- SupplierBoster Bio
- Product NameAnti-SOD2 Antibody
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- Applications SupplierIHP, ICC, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID6648
- Target nameSOD2
- Target descriptionsuperoxide dismutase 2
- Target synonymsGC1, GClnc1, IPO-B, IPOB, MNSOD, MVCD6, Mn-SOD, lncRNA-GC1, superoxide dismutase [Mn], mitochondrial, Mn superoxide dismutase, epididymis secretory sperm binding protein, gastric cancer-associated lncRNA 1, indophenoloxidase B, manganese-containing superoxide dismutase, mangano-superoxide dismutase, superoxide dismutase 2, mitochondrial
- HostRabbit
- IsotypeIgG
- Protein IDP04179
- Protein NameSuperoxide dismutase [Mn], mitochondrial
- Scientific DescriptionBoster Bio Anti-SOD2 Antibody catalog # PA1776. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Mouse, Rat
- Reactivity SupplierHuman, Mouse, Rat, Hamster
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
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- Liu N, Fan X, Shao Y, et al. Resveratrol attenuates inflammation and fibrosis in rheumatoid arthritis-associated interstitial lung disease via the AKT/TMEM175 pathway. J Transl Med. 2024,22(1):457. doi: 10.1186/s12967-024-05228-1Read this paper
- Lin F, Zhang S, Zhu X, et al. Autophagy-related 7 proteindependent autophagy mediates resveratrol-caused upregulation of mitochondrial biogenesis and steroidogenesis in aged Leydig cell. Mol Biol Rep. 2023,51(1):28. doi: 10.1007/s11033-023-08935-yRead this paper
- Bao L, Ye J, Liu N, et al. Resveratrol Ameliorates Fibrosis in Rheumatoid Arthritis-Associated Interstitial Lung Disease via the Autophagy-Lysosome Pathway. Molecules. 2022,27(23). doi: 10.3390/molecules27238475Read this paper
- Guo J, Yu X, Liu Y, et al. Prenatal hypothyroidism diminished exogenous NO-mediated diastolic effects in fetal rat thoracic aorta smooth muscle via increased oxidative stress. Reprod Toxicol. 2022,113:52-61. doi: 10.1016/j.reprotox.2022.08.009Read this paper
- Yao J, Long H, Zhao J, et al. Nifedipine inhibits oxidative stress and ameliorates osteoarthritis by activating the nuclear factor erythroid-2-related factor 2 pathway. Life Sci. 2020,253:117292. doi: 10.1016/j.lfs.2020.117292Read this paper
- Guo S, Zhang YY, Peng JJ, et al. Natural compound methyl protodioscin protects rat brain from ischemia/reperfusion injury through regulation of Mul1/SOD2 pathway. Eur J Pharmacol. 2019,849:50-58. doi: 10.1016/j.ejphar.2019.01.057Read this paper
- Wang T, Wang G, Zhang Y, et al. Effect of lentivirus-mediated overexpression or silencing of MnSOD on apoptosis of resveratrol-treated fibroblast-like synoviocytes in rheumatoid arthritis. Eur J Pharmacol. 2019,844:65-72. doi: 10.1016/j.ejphar.2018.12.001Read this paper
- Dong WT, Xiao LF, Hu JJ, et al. iTRAQ proteomic analysis of the interactions between Bombyx mori nuclear polyhedrosis virus and silkworm. J Proteomics. 2017,166:138-145. doi: 10.1016/j.jprot.2017.07.013Read this paper
- Huang D, Wei W, Xie F, et al. Steroidogenesis decline accompanied with reduced antioxidation and endoplasmic reticulum stress in mice testes during ageing. Andrologia. 2018,50(1). doi: 10.1111/and.12816Read this paper
Datasheet
MSDS
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