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Immunohistochemical staining of human cerebral cortex, colon, lymph node and testis using Anti-SORBS1 antibody HPA027559 (A) shows similar protein distribution across tissues to independent antibody HPA043084 (B).
Immunohistochemical staining of human cerebral cortex, colon, lymph node and testis using Anti-SORBS1 antibody HPA027559 (A) shows similar protein distribution across tissues to independent antibody HPA043084 (B).
Immunohistochemical staining of human cerebral cortex, colon, lymph node and testis using Anti-SORBS1 antibody HPA027559 (A) shows similar protein distribution across tissues to independent antibody HPA043084 (B).

Anti-SORBS1 Antibody

HPA027559
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetSORBS1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SORBS1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10580
  • Target name
    SORBS1
  • Target description
    sorbin and SH3 domain containing 1
  • Target synonyms
    CAP; c-Cbl associated protein; Fas-ligand associated factor 2; FLAF2; ponsin; R85FL; SH3 domain protein 5; SH3D5; SH3P12; SORB1; sorbin and SH3 domain-containing protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BX66
  • Protein Name
    Sorbin and SH3 domain-containing protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human cerebral cortex, colon, lymph node and testis using Anti-SORBS1 antibody HPA043084 (A) shows similar protein distribution across tissues to independent antibody HPA027559 (B).
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Figure 1. Western blot analysis of SORBS1 using anti-SORBS1 antibody (A04426-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SORBS1 antigen affinity purified polyclonal antibody (Catalog # A04426-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SORBS1 at approximately 160KD. The expected band size for SORBS1 is at 142KD.
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Anti-SORBS1 Picoband(r) AntibodyA04426-3-CARRIER-FREE
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