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SORBS1 / Ponsin Antibody

LS-C753817
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSORBS1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SORBS1 / Ponsin Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10580
  • Target name
    SORBS1
  • Target description
    sorbin and SH3 domain containing 1
  • Target synonyms
    CAP, FLAF2, R85FL, SH3D5, SH3P12, SORB1, sorbin and SH3 domain-containing protein 1, Fas-ligand associated factor 2, SH3 domain protein 5, c-Cbl associated protein, ponsin
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SORBS1 using anti-SORBS1 antibody (A04426-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SORBS1 antigen affinity purified polyclonal antibody (Catalog # A04426-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SORBS1 at approximately 160KD. The expected band size for SORBS1 is at 142KD.
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