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IHC-P analysis of human skeletal muscle using GTX24551 SORBS1 antibody, C-term. Antigen retrieval : citrate buffer Ph 6 Dilution : 2.5microg/ml
IHC-P analysis of human skeletal muscle using GTX24551 SORBS1 antibody, C-term. Antigen retrieval : citrate buffer Ph 6 Dilution : 2.5microg/ml
IHC-P analysis of human skeletal muscle using GTX24551 SORBS1 antibody, C-term. Antigen retrieval : citrate buffer Ph 6 Dilution : 2.5microg/ml

SORBS1 antibody, C-term

GTX24551
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSORBS1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SORBS1 antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 2.5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10580
  • Target name
    SORBS1
  • Target description
    sorbin and SH3 domain containing 1
  • Target synonyms
    CAP; c-Cbl associated protein; Fas-ligand associated factor 2; FLAF2; ponsin; R85FL; SH3 domain protein 5; SH3D5; SH3P12; SORB1; sorbin and SH3 domain-containing protein 1
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9BX66
  • Protein Name
    Sorbin and SH3 domain-containing protein 1
  • Scientific Description
    This gene encodes a CBL-associated protein which functions in the signaling and stimulation of insulin. Mutations in this gene may be associated with human disorders of insulin resistance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SORBS1 using anti-SORBS1 antibody (A04426-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SORBS1 antigen affinity purified polyclonal antibody (Catalog # A04426-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SORBS1 at approximately 160KD. The expected band size for SORBS1 is at 142KD.
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