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Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity.
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity.
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity.

Anti-SORBS3 Antibody

HPA015849
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSORBS3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SORBS3
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10174
  • Target name
    SORBS3
  • Target description
    sorbin and SH3 domain containing 3
  • Target synonyms
    SCAM1; SCAM-1; SH3D4; sorbin and SH3 domain-containing protein 3; vinexin; vinexin beta (SH3-containing adaptor molecule-1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60504
  • Protein Name
    Vinexin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SORBS3 using anti-SORBS3 antibody (A05794). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SORBS3 antigen affinity purified polyclonal antibody (Catalog # A05794) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SORBS3 at approximately 87KD. The expected band size for SORBS3 is at 87KD.
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