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Western blot All lanes: SORBS3 antibody at 8microg/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 76, 37 kDa Observed band size: 76 kDa
Western blot All lanes: SORBS3 antibody at 8microg/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 76, 37 kDa Observed band size: 76 kDa
Western blot All lanes: SORBS3 antibody at 8microg/ml + HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 76, 37 kDa Observed band size: 76 kDa

SORBS3 Antibody

CSB-PA022406LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSORBS3
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Overview

  • Supplier
    Cusabio
  • Product Name
    SORBS3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10174
  • Target name
    SORBS3
  • Target description
    sorbin and SH3 domain containing 3
  • Target synonyms
    SCAM-1, SCAM1, SH3D4, vinexin, sorbin and SH3 domain-containing protein 3, vinexin beta (SH3-containing adaptor molecule-1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60504
  • Protein Name
    Vinexin
  • Scientific Description
    Vinexin alpha isoform promotes up-regulation of actin stress fiber formation. Vinexin beta isoform plays a role in cell spreading and enhances the activation of JNK/SAPK in response to EGF stimulation by using its third SH3 domain.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SORBS3 using anti-SORBS3 antibody (A05794). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SORBS3 antigen affinity purified polyclonal antibody (Catalog # A05794) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SORBS3 at approximately 87KD. The expected band size for SORBS3 is at 87KD.
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TargetSORBS3
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TargetSORBS3
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