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Anti-SOS1 Antibody

144-03272
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSOS1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SOS1 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6654
  • Target name
    SOS1
  • Target description
    SOS Ras/Rac guanine nucleotide exchange factor 1
  • Target synonyms
    GF1, GGF1, GINGF, HGF, NS4, SOS-1, son of sevenless homolog 1, gingival fibromatosis, hereditary, 1, guanine nucleotide exchange factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07889
  • Protein Name
    Son of sevenless homolog 1
  • Scientific Description
    SOS1 Polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SOS1 using anti-SOS1 antibody (A00837-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOS1 antigen affinity purified polyclonal antibody (Catalog # A00837-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOS1 at approximately 170 kDa. The expected band size for SOS1 is at 152 kDa.
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