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SOS1 antibody detects SOS1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SOS1 stained by SOS1 antibody (GTX133774) diluted at 1:500. Blue: Hoechst 33342 staining.
SOS1 antibody detects SOS1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SOS1 stained by SOS1 antibody (GTX133774) diluted at 1:500. Blue: Hoechst 33342 staining.
SOS1 antibody detects SOS1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SOS1 stained by SOS1 antibody (GTX133774) diluted at 1:500. Blue: Hoechst 33342 staining.

SOS1 antibody

GTX133774
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetSOS1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SOS1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.58 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6654
  • Target name
    SOS1
  • Target description
    SOS Ras/Rac guanine nucleotide exchange factor 1
  • Target synonyms
    GF1, GGF1, GINGF, HGF, NS4, SOS-1, son of sevenless homolog 1, gingival fibromatosis, hereditary, 1, guanine nucleotide exchange factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07889
  • Protein Name
    Son of sevenless homolog 1
  • Scientific Description
    This gene encodes a protein that is a guanine nucleotide exchange factor for RAS proteins, membrane proteins that bind guanine nucleotides and participate in signal transduction pathways. GTP binding activates and GTP hydrolysis inactivates RAS proteins. The product of this gene may regulate RAS proteins by facilitating the exchange of GTP for GDP. Mutations in this gene are associated with gingival fibromatosis 1 and Noonan syndrome type 4. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SOS1 using anti-SOS1 antibody (A00837-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOS1 antigen affinity purified polyclonal antibody (Catalog # A00837-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOS1 at approximately 170 kDa. The expected band size for SOS1 is at 152 kDa.
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