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Immunohistochemical staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human Testis shows moderate nuclear positivity in cells in seminiferous ducts.

Anti-SP7 Antibody

HPA063202
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSP7
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SP7
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID121340
  • Target name
    SP7
  • Target description
    Sp7 transcription factor
  • Target synonyms
    OI11; OI12; osterix; OSX; transcription factor Sp7; zinc finger protein osterix
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TDD2
  • Protein Name
    Transcription factor Sp7
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Sp7/Osterix using anti-Sp7/Osterix antibody (A02077-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human MDA-MB453 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human THP-1 whole cell lysates. Lane 7: rat lung tissue lysates, Lane 8: rat thymus tissue lysates, Lane 9: rat skeletal muscle tissue lysates, Lane 10: rat testis tissue lysates, Lane 11: mouse lung tissue lysates, Lane 12: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sp7/Osterix antigen affinity purified polyclonal antibody (Catalog # A02077-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sp7/Osterix at approximately 45KD. The expected band size for Sp7/Osterix is at 45KD.
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