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SP7 antibody

GTX129385
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetSP7
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Overview

  • Supplier
    GeneTex
  • Product Name
    SP7 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID121340
  • Target name
    SP7
  • Target description
    Sp7 transcription factor
  • Target synonyms
    OI11; OI12; osterix; OSX; transcription factor Sp7; zinc finger protein osterix
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TDD2
  • Protein Name
    Transcription factor Sp7
  • Scientific Description
    SP7 is a C2H2-type zinc finger transcription factor of the SP gene family and a putative master regulator of bone cell differentiation (Gao et al., 2004 [PubMed 15474293]).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Sp7/Osterix using anti-Sp7/Osterix antibody (A02077-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human MDA-MB453 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human THP-1 whole cell lysates. Lane 7: rat lung tissue lysates, Lane 8: rat thymus tissue lysates, Lane 9: rat skeletal muscle tissue lysates, Lane 10: rat testis tissue lysates, Lane 11: mouse lung tissue lysates, Lane 12: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sp7/Osterix antigen affinity purified polyclonal antibody (Catalog # A02077-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sp7/Osterix at approximately 45KD. The expected band size for Sp7/Osterix is at 45KD.
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