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Mouse spinal cord lysates probed with Osterix Polyclonal Antibody, unconjugated (bs-1110R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Mouse spinal cord lysates probed with Osterix Polyclonal Antibody, unconjugated (bs-1110R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Mouse spinal cord lysates probed with Osterix Polyclonal Antibody, unconjugated (bs-1110R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

SP7/Osterix Polyclonal Antibody

BS-1110R
Bioss
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSP7
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Overview

  • Supplier
    Bioss
  • Product Name
    SP7/Osterix Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID121340
  • Target name
    SP7
  • Target description
    Sp7 transcription factor
  • Target synonyms
    OI11, OI12, OSX, osterix, transcription factor Sp7, zinc finger protein osterix
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TDD2
  • Protein Name
    Transcription factor Sp7
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Chang Z, Yan G, Yan H, et al. Reticulocalbin 2 enhances osteogenic differentiation of human vascular smooth muscle cells in diabetic conditions. Life Sci. 2019,233:116746. doi: 10.1016/j.lfs.2019.116746
    Read this paper
  • Cui Q, Xing J, Yu M, et al. Mmu-miR-185 depletion promotes osteogenic differentiation and suppresses bone loss in osteoporosis through the Bgn-mediated BMP/Smad pathway. Cell Death Dis. 2019,10(3):172. doi: 10.1038/s41419-019-1428-1
    Read this paper
  • Bihari C, Lal D, Thakur M, et al. Suboptimal Level of Bone-Forming Cells in Advanced Cirrhosis are Associated with Hepatic Osteodystrophy. Hepatol Commun. 2018,2(9):1095-1110. doi: 10.1002/hep4.1234
    Read this paper
  • Qian DY, Yan GB, Bai B, et al. Differential circRNA expression profiles during the BMP2-induced osteogenic differentiation of MC3T3-E1 cells. Biomed Pharmacother. 2017,90:492-499. doi: 10.1016/j.biopha.2017.03.051
    Read this paper
  • Li S, Xu W, Xing Z, et al. A Conditional Knockout Mouse Model Reveals a Critical Role of PKD1 in Osteoblast Differentiation and Bone Development. Sci Rep. 2017,7:40505. doi: 10.1038/srep40505
    Read this paper
  • Ranganathan K, Agarwal S, Cholok D, et al. The role of the adaptive immune system in burn-induced heterotopic ossification and mesenchymal cell osteogenic differentiation. J Surg Res. 2016,206(1):53-61. doi: 10.1016/j.jss.2016.04.040
    Read this paper
  • Rajshankar D, Wang Y, McCulloch CA. Osteogenesis requires FAK-dependent collagen synthesis by fibroblasts and osteoblasts. FASEB J. 2017,31(3):937-953. doi: 10.1096/fj.201600645R
    Read this paper
  • Wang Y, He T, Liu J, et al. Synergistic effects of overexpression of BMP‑2 and TGF‑β3 on osteogenic differentiation of bone marrow mesenchymal stem cells. Mol Med Rep. 2016,14(6):5514-5520. doi: 10.3892/mmr.2016.5961
    Read this paper
  • Tang Z, Tan Y, Ni Y, et al. Comparison of ectopic bone formation process induced by four calcium phosphate ceramics in mice. Mater Sci Eng C Mater Biol Appl. 2017,70(Pt 2):1000-1010. doi: 10.1016/j.msec.2016.06.097
    Read this paper
  • Agarwal S, Loder S, Cholok D, et al. Local and Circulating Endothelial Cells Undergo Endothelial to Mesenchymal Transition (EndMT) in Response to Musculoskeletal Injury. Sci Rep. 2016,6:32514. doi: 10.1038/srep32514
    Read this paper

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Figure 1. Western blot analysis of Sp7/Osterix using anti-Sp7/Osterix antibody (A02077-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human MDA-MB453 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human THP-1 whole cell lysates. Lane 7: rat lung tissue lysates, Lane 8: rat thymus tissue lysates, Lane 9: rat skeletal muscle tissue lysates, Lane 10: rat testis tissue lysates, Lane 11: mouse lung tissue lysates, Lane 12: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sp7/Osterix antigen affinity purified polyclonal antibody (Catalog # A02077-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sp7/Osterix at approximately 45KD. The expected band size for Sp7/Osterix is at 45KD.
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