Anti-Spike protein [4A8]

Absolute Antibody

Anti-Spike protein [4A8]

9

This antibody is specific for S1 and S-ECD of the SARS CoV2 spike protein. SARS-CoV-2 is a positive-sense single-stranded RNA virus[5] that is contagious in humans.

The binding profile of this antibody was determined by performing an ELISA on SARS-CoV-2 S, S1, S2 or RBD protein using the human version of this antibody. Furthermore a neutralization experiment on Vero-E6 cells infected with SARS CoV2 was done using the human IgG1 version of this antibody. The binding of the human version was also characterized using biolayer interferometry. A flow cytometric receptor binding inhibition assay was also performed on the human version of this antibody incubated in SARS-CoV-2S protein and then incubated with ACE2-293T cells. The complex of S-ECD and the human version of this antibody was characterized using cryo electromicroscopy (Chi et al, 2020; pmid:32571838).

Functional Assay, Flow Cytometry, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

4A8

S

surface glycoprotein

GU280_gp02; spike glycoprotein; surface glycoprotein

Human

IgG2

Spike glycoprotein

This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203