Anti-Spike protein [EY6A]

Absolute Antibody

Anti-Spike protein [EY6A]

9

This antibody binds tightly (KD of 2 nM) to the receptor binding domain (RBD) the of the SARS-CoV-2. It also cross reacts with SARS-CoV-1. This antibody recognizes a highly conserved epitope on away from the ACE2 receptor binding domain.

EY6A binds S1 domain of SARS-CoV-2 and also cross reacts with SARS-CoV-1. The binding was confirmed by ELISA. Further confirmation of binding of EY6A to SARS-CoV-2 infected cells was done by immunofluorescence. Surface plasmon resonance (SPR) measurements for EY6A Fab showed high affinity binding to immobilised SARS-CoV-2 RBD (KD = 2 nM). EY6A did not completely block binding of ACE2 to RBD, but was successful in neutralizing SARS-CoV-2 infection in Vero E6 cells (Zhou et al., 2020).

ImmunoFluorescence, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

EY6A

S

surface glycoprotein

GU280_gp02; spike glycoprotein; surface glycoprotein

Human

IgG1

Spike glycoprotein

Virus

-20°C,2°C to 8°C

12352203