Anti-Spike protein [MR3]

Absolute Antibody

Anti-Spike protein [MR3]

9

This antibody binds the receptor binding domain (RBD) of the SARS-CoV-2.

MR3 is a potent antibody that binds the RBD with an affiinty of KD=1.0 nM and neutralizes SARS-CoV-2 pseudovirus with an IC50 of 0.40 microg m/L. Consistent with its highest affinity, MR3 showed the slowest off-rate (2.3 x 10-4 per second). ELISA was used to screen the libraries to identify specific RBD binders. Neutralization activity of the sybody was checked by pre-incubating pseudoviral particles with different concentrations of sybody before infection of VeroE6-hACE2 cells. The rate of infection was then measured by fluorescence-activated cell sorting (FACS). The binding kinetics between sybodies and the RBD was assessed using bio-layer interferometry (Li et al., 2020).

Flow Cytometry, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

MR3

S

surface glycoprotein

GU280_gp02; spike glycoprotein; surface glycoprotein

Human

IgM

Spike glycoprotein

This chimeric human antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203