Anti-Spike protein [Sb23]

Absolute Antibody

Anti-Spike protein [Sb23]

9

This antibody is capable of binding both recombinant RBD as well as to the pre-fusion spike glycoprotein with high affinity. Sb23 binds an epitope in the ACE2 binding site on the RBD in both its up and down conformation and thereby effectively blocks ACE2

Sb23 binds RBD with a very high affinity and neutralizes pseudovirus with an IC50 of 0.6 microg/ml. Sb23 and ACE2 compete for the same or overlapping binding sites on SARS-CoV-2-RBD. ELISA was used to screen the libraries to identify specific RBD binders. It is also reported that since Sb23 can efficiently replace ACE2 from a preformed complex, it has a higher affinity for RBD than ACE2. Biolayer interferometry assay was used to kinetically characterize the interaction of sybodies with RBD and spike (Custodio et al., 2020).

ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

Sb23

S

surface glycoprotein

GU280_gp02; spike glycoprotein; surface glycoprotein

Rabbit

IgG

Spike glycoprotein

This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203