Anti-Spike protein [SR4]

Absolute Antibody

Anti-Spike protein [SR4]

9

This antibody binds the receptor binding domain (RBD) of the SARS-CoV-2.

Structural alignment of the SR4-RBD complex and ACE2-RBD complex revealed that binding of SR4 did not cause any noticable conformation changes of the RBD. ELISA was used to screen the libraries to identify specific RBD binders. Neutralization activity of the sybody was checked by pre-incubating pseudoviral particles with different concentrations of sybody before infection of VeroE6-hACE2 cells. The rate of infection was then measured by fluorescence-activated cell sorting (FACS). IC50 value of SR4 was determined to be 5.90 microg m/L. The binding kinetics between sybodies and the RBD was assessed using bio-layer interferometry (Li et al., 2020).

Flow Cytometry, ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

SR4

S

surface glycoprotein

GU280_gp02; spike glycoprotein; surface glycoprotein

Mouse

IgG1

Spike glycoprotein

This chimeric mouse antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203