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Anti-SPTAN1 Antibody

144-60011
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetSPTAN1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-SPTAN1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6709
  • Target name
    SPTAN1
  • Target description
    spectrin alpha, non-erythrocytic 1
  • Target synonyms
    alpha-fodrin; alpha-II spectrin; DEE5; EIEE5; epididymis secretory sperm binding protein; fodrin alpha chain; NEAS; spectrin alpha chain, non-erythrocytic 1; spectrin, non-erythroid alpha chain; spectrin, non-erythroid alpha subunit; SPTA2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13813
  • Protein Name
    Spectrin alpha chain, non-erythrocytic 1
  • Scientific Description
    SPTAN1 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NEAS/SPTAN1 using anti-NEAS/SPTAN1 antibody (A03831-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEAS/SPTAN1 antigen affinity purified polyclonal antibody (Catalog # A03831-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEAS/SPTAN1 at approximately 285 kDa. The expected band size for NEAS/SPTAN1 is at 285 kDa.
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