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ProductsBack/NEAS/alpha-Fodrin Antibody (YA1355, Rabbit)

NEAS/alpha-Fodrin Antibody (YA1355, Rabbit)

HY-P81610
MedChem Express
TargetSPTAN1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    NEAS/alpha-Fodrin Antibody (YA1355, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6709
  • Target name
    SPTAN1
  • Target description
    spectrin alpha, non-erythrocytic 1
  • Target synonyms
    DEE5, DEVEP, EIEE5, HMN11, HMND11, NEAS, SPG91, SPTA2, spectrin alpha chain, non-erythrocytic 1, alpha-II spectrin, alpha-fodrin, epididymis secretory sperm binding protein, fodrin alpha chain, spectrin, non-erythroid alpha chain, spectrin, non-erythroid alpha subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    NEAS/alpha-Fodrin Antibody (YA1355) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NEAS/alpha-Fodrin.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NEAS/SPTAN1 using anti-NEAS/SPTAN1 antibody (A03831-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEAS/SPTAN1 antigen affinity purified polyclonal antibody (Catalog # A03831-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEAS/SPTAN1 at approximately 285 kDa. The expected band size for NEAS/SPTAN1 is at 285 kDa.
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