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Immunohistochemical staining of human spleen shows moderate cytoplasmic positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows moderate cytoplasmic positivity in cells in red pulp.
Immunohistochemical staining of human spleen shows moderate cytoplasmic positivity in cells in red pulp.

Anti-STAT3-25ul

HPA058603
Atlas Antibodies
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-STAT3-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
  • Applications Supplier
    IHC, WB, ChIP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP40763
  • Protein Name
    Signal transducer and activator of transcription 3
  • Scientific Description
    Rabbit Polyclonal Anti-STAT3 Antibody against Human signal transducer and activator of transcription 3 (acute-phase response factor). Validated for Chromatin Immunoprecipitation, Immunohistochemistry and Western Blot
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STAT3 using anti-STAT3 antibody (PB9511). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human SiHa whole cell lysates, Lane 7: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 antigen affinity purified polyclonal antibody (Catalog # PB9511) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for STAT3 at approximately 88 kDa. The expected band size for STAT3 is at 88 kDa.
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