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Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Anti-STRADB Antibody

HPA028520
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetSTRADB
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-STRADB
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55437
  • Target name
    STRADB
  • Target description
    STE20 related adaptor beta
  • Target synonyms
    ALS2CR2; amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2; amyotrophic lateral sclerosis 2 chromosomal region candidate gene 2 protein; CALS-21; ILP-interacting protein ILPIPA; ILPIP; ILPIPA; PAPK; PRO1038; pseudokinase ALS2CR2; STE20-related kinase adapter protein beta; STE20-related kinase adaptor beta; STRAD beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9C0K7
  • Protein Name
    STE20-related kinase adapter protein beta
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of STRADB using anti-STRADB antibody (A05271-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STRADB antigen affinity purified polyclonal antibody (Catalog # A05271-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STRADB at approximately 33 kDa. The expected band size for STRADB is at 47 kDa.
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SH-SY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with PAPK/ALS2CR2 Polyclonal Antibody(bs-3928R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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