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Anti-SUCLA2 Antibody

Anti-SUCLA2 Antibody

A12768
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-SUCLA2 Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to SUCLA2.
  • UNSPSC
    12352203

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Western blot All lanes: SUCLA2 antibody at 1.75microg/ml Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: A549 whole cell lysate Lane 4: 293T whole cell lysate Lane 5: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51, 49 kDa Observed band size: 51 kDa
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Immunohistochemical staining of human cerebral cortex, colon, kidney and lymph node using Anti-SUCLA2 antibody HPA039536 (A) shows similar protein distribution across tissues to independent antibody HPA039435 (B).
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Figure 1. Western blot analysis of SUCLA2 using anti-SUCLA2 antibody (A04807-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUCLA2 antigen affinity purified polyclonal antibody (Catalog # A04807-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUCLA2 at approximately 48 kDa. The expected band size for SUCLA2 is at 48 kDa.
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