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Anti-SUFU Antibody

Anti-SUFU Antibody

A326304
Antibodies.com
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-SUFU Antibody
  • Delivery Days Customer
    7
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Host
    Goat
  • Isotype
    IgG
  • Scientific Description
    Goat polyclonal antibody to SUFU.
  • Reactivity
    Human
  • UNSPSC
    12352203

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Figure 2. IF analysis of SUFU using anti-SUFU antibody (A02279-1). SUFU was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2microg/mL rabbit anti-SUFU Antibody (A02279-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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TargetSUFU
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Anti-SUFU Antibody144-06757
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Lane 1:Mouse intestine lysate Lane 2: Mouse lung lysate Lane 3: Mouse brain probed with Rabbit Anti-SUFU/Suppressor of Fused Polyclonal Antibody, Unconjugated (bs-11209R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
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Bioss
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ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
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LifeSpan BioSciences
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Rat whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Suppressor of Fused antibody [HL3374] (GTX641194) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GeneTex
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TargetSUFU
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Immunohistochemical staining of human Testis shows strong nuclear positivity in cells in seminiferous ducts.
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Western blot All lanes: Suppressor of fused homolog antibody at 4ug/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 54, 48, 53 kDa Observed band size: 54 kDa
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