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SUFU Antibody

LS-C748477
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetSUFU
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SUFU Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 47kDa/52kDa/53kDa, while the observed MW by Western blot was 54kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 47kDa/52kDa/53kDa, while the observed MW by Western blot was 54kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51684
  • Target name
    SUFU
  • Target description
    SUFU negative regulator of hedgehog signaling
  • Target synonyms
    JBTS32; negative regulator of hedgehog signaling; PRO1280; SUFUH; SUFUXL; suppressor of fused homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 2. IF analysis of SUFU using anti-SUFU antibody (A02279-1). SUFU was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2microg/mL rabbit anti-SUFU Antibody (A02279-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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