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SUFU Antibody

LS-C748477
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSUFU
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SUFU Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 47kDa/52kDa/53kDa, while the observed MW by Western blot was 54kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 47kDa/52kDa/53kDa, while the observed MW by Western blot was 54kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51684
  • Target name
    SUFU
  • Target description
    SUFU negative regulator of hedgehog signaling
  • Target synonyms
    BCNS2, JBTS32, PRO1280, SUFUH, SUFUXL, suppressor of fused homolog, negative regulator of hedgehog signaling
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 2. IF analysis of SUFU using anti-SUFU antibody (A02279-1). SUFU was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2microg/mL rabbit anti-SUFU Antibody (A02279-1) overnight at 4°C. DyLight®488 conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Lane 1:Mouse intestine lysate Lane 2: Mouse lung lysate Lane 3: Mouse brain probed with Rabbit Anti-SUFU/Suppressor of Fused Polyclonal Antibody, Unconjugated (bs-11209R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
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Western blot All lanes: Suppressor of fused homolog antibody at 4ug/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 54, 48, 53 kDa Observed band size: 54 kDa
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Rat whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Suppressor of Fused antibody [HL3374] (GTX641194) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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