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Immunohistochemical staining of human testis shows distinct nuclear membranous and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows distinct nuclear membranous and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows distinct nuclear membranous and cytoplasmic positivity in cells in seminiferous ducts.

Anti-SYNE3 Antibody

HPA055227
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSYNE3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-SYNE3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID161176
  • Target name
    SYNE3
  • Target description
    spectrin repeat containing nuclear envelope family member 3
  • Target synonyms
    C14orf139, C14orf49, KASH3, LINC00341, NCRNA00341, NET53, Nesp3, nesprin-3, KASH domain-containing protein 3, long intergenic non-protein coding RNA 341, nuclear envelope spectrin repeat protein 3, uncharacterized protein C14orf113
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6ZMZ3
  • Protein Name
    Nesprin-3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of Nesprin3/SYNE3 using anti-Nesprin3/SYNE3 antibody (A13164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nesprin3/SYNE3 antigen affinity purified polyclonal antibody (Catalog # A13164-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nesprin3/SYNE3 at approximately 100 kDa. The expected band size for Nesprin3/SYNE3 is at 112 kDa.
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