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ProductsBack/SYNE3 / C14orf49 Antibody

SYNE3 / C14orf49 Antibody

LS-C748298
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetSYNE3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SYNE3 / C14orf49 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 68kDa/111kDa/112kDa, while the observed MW by Western blot was 112kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 68kDa/111kDa/112kDa, while the observed MW by Western blot was 112kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID161176
  • Target name
    SYNE3
  • Target description
    spectrin repeat containing nuclear envelope family member 3
  • Target synonyms
    C14orf139, C14orf49, KASH3, LINC00341, NCRNA00341, NET53, Nesp3, nesprin-3, KASH domain-containing protein 3, long intergenic non-protein coding RNA 341, nuclear envelope spectrin repeat protein 3, uncharacterized protein C14orf113
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nesprin3/SYNE3 using anti-Nesprin3/SYNE3 antibody (A13164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nesprin3/SYNE3 antigen affinity purified polyclonal antibody (Catalog # A13164-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nesprin3/SYNE3 at approximately 100 kDa. The expected band size for Nesprin3/SYNE3 is at 112 kDa.
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