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WB analysis of Jurkat cell lysates using GTX87974 Nesprin 3 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of Jurkat cell lysates using GTX87974 Nesprin 3 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of Jurkat cell lysates using GTX87974 Nesprin 3 antibody. The lane on the right is blocked with the synthesized peptide.

Nesprin 3 antibody

GTX87974
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetSYNE3
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Overview

  • Supplier
    GeneTex
  • Product Name
    Nesprin 3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID161176
  • Target name
    SYNE3
  • Target description
    spectrin repeat containing nuclear envelope family member 3
  • Target synonyms
    C14orf139, C14orf49, KASH3, LINC00341, NCRNA00341, NET53, Nesp3, nesprin-3, KASH domain-containing protein 3, long intergenic non-protein coding RNA 341, nuclear envelope spectrin repeat protein 3, uncharacterized protein C14orf113
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6ZMZ3
  • Protein Name
    Nesprin-3
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Hobbs RP, DePianto DJ, Jacob JT, et al. Keratin-dependent regulation of Aire and gene expression in skin tumor keratinocytes. Nat Genet. 2015,47(8):933-8. doi: 10.1038/ng.3355
    Read this paper
  • Shaiken TE, Opekun AR. Dissecting the cell to nucleus, perinucleus and cytosol. Sci Rep. 2014,4:4923. doi: 10.1038/srep04923
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Nesprin3/SYNE3 using anti-Nesprin3/SYNE3 antibody (A13164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nesprin3/SYNE3 antigen affinity purified polyclonal antibody (Catalog # A13164-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nesprin3/SYNE3 at approximately 100 kDa. The expected band size for Nesprin3/SYNE3 is at 112 kDa.
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