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Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.

Anti-TAB3 Antibody

HPA034980
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTAB3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TAB3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID257397
  • Target name
    TAB3
  • Target description
    TGF-beta activated kinase 1 (MAP3K7) binding protein 3
  • Target synonyms
    MAP3K7IP3, NAP1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 3, NF-kappa-B-activating protein 1, NFkB activating protein 1, TAB-3, TAK1-binding protein 3, TGF-beta activated kinase 1 and MAP3K7 binding protein 3, TGF-beta-activated kinase 1-binding protein 3, mitogen-activated protein kinase kinase kinase 7 interacting protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N5C8
  • Protein Name
    TGF-beta-activated kinase 1 and MAP3K7-binding protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TAB3 using anti-TAB3 antibody (A05084-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAB3 antigen affinity purified polyclonal antibody (Catalog # A05084-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAB3 at approximately 90 kDa. The expected band size for TAB3 is at 79 kDa.
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