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Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.

Anti-TEAD1-25ul

HPA057339
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TEAD1-25ul
  • Delivery Days Customer
    6
  • Applications
    ImmunoCytoChemistry
  • Applications Supplier
    ICC
  • Category Supplier
    Antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.05
  • Conjugate
    Unconjugated
  • Gene ID
    P28347
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP28347
  • Protein Name
    Transcriptional enhancer factor TEF-1
  • Scientific Description
    Rabbit Polyclonal Anti-TEAD1 Antibody against Human TEA domain family member 1 (SV40 transcriptional enhancer factor). Validated for Immunofluorescence
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TEF1/TEAD-1/TEAD1 using anti-TEF1/TEAD-1/TEAD1 antibody (A03263-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEF1/TEAD-1/TEAD1 antigen affinity purified polyclonal antibody (Catalog # A03263-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEF1/TEAD-1/TEAD1 at approximately 50KD. The expected band size for TEF1/TEAD-1/TEAD1 is at 50KD.
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