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TEAD1 Antibody

CAB13366
Assay Genie
ReactivityHuman
Product group Antibodies
TargetTEAD1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    TEAD1 Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC,IF,ChIP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID7003
  • Target name
    TEAD1
  • Target description
    TEA domain transcription factor 1
  • Target synonyms
    AA, NTEF-1, REF1, TCF-13, TCF13, TEAD-1, TEF-1, transcriptional enhancer factor TEF-1, TEA domain family member 1 (SV40 transcriptional enhancer factor), protein GT-IIC, transcription factor 13, transcriptional enhancer factor 1
  • Host
    Rabbit
  • Protein IDP28347
  • Protein Name
    Transcriptional enhancer factor TEF-1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate All lanes: TEAD1 antibody at 3.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 48 kDa Observed band size: 48 kDa
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Figure 1. Western blot analysis of TEF1/TEAD-1/TEAD1 using anti-TEF1/TEAD-1/TEAD1 antibody (A03263-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TEF1/TEAD-1/TEAD1 antigen affinity purified polyclonal antibody (Catalog # A03263-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TEF1/TEAD-1/TEAD1 at approximately 50KD. The expected band size for TEF1/TEAD-1/TEAD1 is at 50KD.
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HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TEF1 antibody [GT13112] (GTX632126) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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