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Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.

Anti-TGFBR3 Antibody

HPA008257
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTGFBR3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TGFBR3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7049
  • Target name
    TGFBR3
  • Target description
    transforming growth factor beta receptor 3
  • Target synonyms
    BGCAN, betaglycan, transforming growth factor beta receptor type 3, TGF-beta receptor type 3, TGF-beta receptor type III, TGFR-3, betaglycan proteoglycan, transforming growth factor beta receptor III
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03167
  • Protein Name
    Transforming growth factor beta receptor type 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TGF Beta Receptor III/TGFBR3 using anti-TGF Beta Receptor III/TGFBR3 antibody (A02765-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat L6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGF Beta Receptor III/TGFBR3 antigen affinity purified polyclonal antibody (Catalog # A02765-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGF Beta Receptor III/TGFBR3 at approximately 93 kDa. The expected band size for TGF Beta Receptor III/TGFBR3 is at 93 kDa.
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Lane 1: K562 Cells; Lane 2: HepG2 Cells; Lane 3: Hela Cells; Probed with TGF Beta R3 Polyclonal Antibody (bs-1910R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Western blot All lanes: TGFBR3 antibody IgG at 2.39ug/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 93 kDa Observed band size: 93 kDa
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