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Western blot All lanes: TGFBR3 antibody IgG at 2.39ug/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 93 kDa Observed band size: 93 kDa
Western blot All lanes: TGFBR3 antibody IgG at 2.39ug/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 93 kDa Observed band size: 93 kDa
Western blot All lanes: TGFBR3 antibody IgG at 2.39ug/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 93 kDa Observed band size: 93 kDa

TGFBR3 Antibody

CSB-PA023453ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTGFBR3
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Overview

  • Supplier
    Cusabio
  • Product Name
    TGFBR3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7049
  • Target name
    TGFBR3
  • Target description
    transforming growth factor beta receptor 3
  • Target synonyms
    betaglycan; betaglycan proteoglycan; BGCAN; TGF-beta receptor type 3; TGF-beta receptor type III; TGFR-3; transforming growth factor beta receptor III; transforming growth factor beta receptor type 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03167
  • Protein Name
    Transforming growth factor beta receptor type 3
  • Scientific Description
    Binds to TGF-beta. Could be involved in capturing and retaining TGF-beta for presentation to the signaling receptors.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TGF Beta Receptor III/TGFBR3 using anti-TGF Beta Receptor III/TGFBR3 antibody (A02765-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat L6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGF Beta Receptor III/TGFBR3 antigen affinity purified polyclonal antibody (Catalog # A02765-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGF Beta Receptor III/TGFBR3 at approximately 93 kDa. The expected band size for TGF Beta Receptor III/TGFBR3 is at 93 kDa.
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