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Tgfbr3 Polyclonal Antibody

CAC10904
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTGFBR3
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Overview

  • Supplier
    Biomatik
  • Product Name
    Tgfbr3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7049
  • Target name
    TGFBR3
  • Target description
    transforming growth factor beta receptor 3
  • Target synonyms
    BGCAN, betaglycan, transforming growth factor beta receptor type 3, TGF-beta receptor type 3, TGF-beta receptor type III, TGFR-3, betaglycan proteoglycan, transforming growth factor beta receptor III
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ03167
  • Protein Name
    Transforming growth factor beta receptor type 3
  • Scientific Description
    The Tgfbr3 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of TGF Beta Receptor III/TGFBR3 using anti-TGF Beta Receptor III/TGFBR3 antibody (A02765-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat L6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TGF Beta Receptor III/TGFBR3 antigen affinity purified polyclonal antibody (Catalog # A02765-4) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TGF Beta Receptor III/TGFBR3 at approximately 93 kDa. The expected band size for TGF Beta Receptor III/TGFBR3 is at 93 kDa.
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Lane 1: K562 Cells; Lane 2: HepG2 Cells; Lane 3: Hela Cells; Probed with TGF Beta R3 Polyclonal Antibody (bs-1910R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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