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Immunohistochemical staining of human lung shows strong membranous positivity in pneumocytes and endothelial cells.
Immunohistochemical staining of human lung shows strong membranous positivity in pneumocytes and endothelial cells.
Immunohistochemical staining of human lung shows strong membranous positivity in pneumocytes and endothelial cells.

Anti-THBD Antibody

HPA002982
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetTHBD
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-THBD
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7056
  • Target name
    THBD
  • Target description
    thrombomodulin
  • Target synonyms
    AHUS6; BDCA3; BDCA-3; CD141; CD141 antigen; fetomodulin; THPH12; THRM; thrombomodulin; TM
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07204
  • Protein Name
    Thrombomodulin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: THBD antibody at 3.07microg/ml Lane 1: Rat heart tissue Lane 2: Mouse kidney tissue Lane 3: A549 whole cell lysate Lane 4: A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60 kDa Observed band size: 60 kDa
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Figure 1. Western blot analysis of Thrombomodulin/THBD using anti-Thrombomodulin/THBD antibody (A01325-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombomodulin/THBD antigen affinity purified polyclonal antibody (Catalog # A01325-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Thrombomodulin/THBD at approximately 100 kDa. The expected band size for Thrombomodulin/THBD is at 60 kDa.
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