Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Thrombomodulin antibody [C3], C-term (GTX108277) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
![Thrombomodulin antibody [C3], C-term detects THBD protein at cytosol and membrane on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. Thrombomodulin antibody [C3], C-term (GTX108277) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX108277/GTX108277_39757_IHC_w_23060120_240.webp "Thrombomodulin antibody [C3], C-term detects THBD protein at cytosol and membrane on human gastric cancer by immunohistochemical analysis. Sample: Paraffin-embedded gastric cancer. Thrombomodulin antibody [C3], C-term (GTX108277) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Thrombomodulin antibody [C3], C-term detects Thrombomodulin protein at junction by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thrombomodulin protein stained by Thrombomodulin antibody [C3], C-term (GTX108277) diluted at 1:200. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX108277/GTX108277_41766_20150629_IFA_w_23060120_670.webp "Thrombomodulin antibody [C3], C-term detects Thrombomodulin protein at junction by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thrombomodulin protein stained by Thrombomodulin antibody [C3], C-term (GTX108277) diluted at 1:200. Blue: Hoechst 33342 staining.")
![Immunoprecipitation of Thrombomodulin protein from THP-1 whole cell extracts using 5 μg of Thrombomodulin antibody [C3], C-term (GTX108277). Western blot analysis was performed using Thrombomodulin antibody [C3], C-term (GTX108277). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX108277/GTX108277_39757_20151204_IP_w_23060120_837.webp "Immunoprecipitation of Thrombomodulin protein from THP-1 whole cell extracts using 5 μg of Thrombomodulin antibody [C3], C-term (GTX108277). Western blot analysis was performed using Thrombomodulin antibody [C3], C-term (GTX108277). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Thrombomodulin antibody [C3], C-term (GTX108277) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Thrombomodulin antibody [C3], C-term
GTX108277
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTHBD
Overview
- SupplierGeneTex
- Product NameThrombomodulin antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7056
- Target nameTHBD
- Target descriptionthrombomodulin
- Target synonymsAHUS6, BDCA-3, BDCA3, CD141, THPH12, THRM, TM, thrombomodulin, CD141 antigen, fetomodulin
- HostRabbit
- IsotypeIgG
- Protein IDP07204
- Protein NameThrombomodulin
- Scientific DescriptionThe protein encoded by this intronless gene is an endothelial-specific type I membrane receptor that binds thrombin. This binding results in the activation of protein C, which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Mutations in this gene are a cause of thromboembolic disease, also known as inherited thrombophilia. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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