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ProductsBack/Anti-Thy-1 [YBM 29.2.1]

Anti-Thy-1 [YBM 29.2.1]

Ab00211-2.0
Absolute Antibody
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetThy1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Thy-1 [YBM 29.2.1]
  • Delivery Days Customer
    7
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    YBM 29.2.1
  • Gene ID21838
  • Target name
    Thy1
  • Target description
    thymus cell antigen 1, theta
  • Target synonyms
    CD90, T25, Thy-1, Thy-1.2, Thy1.1, Thy1.2, thy-1 membrane glycoprotein, thy-1 antigen
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP01831
  • Protein Name
    Thy-1 membrane glycoprotein
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Rat IgG2c format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Mouse
  • Reactivity Supplier Note
    Mouse Thy-1.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD90/Thy1 using anti-CD90/Thy1 antibody (A01818). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rai thymus tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD90/Thy1 antigen affinity purified polyclonal antibody (Catalog # A01818) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD90/Thy1 at approximately 22 kDa. The expected band size for CD90/Thy1 is at 18 kDa.
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