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ProductsBack/Biotin Anti-Mouse CD90 Antibody [M5/49.4.1]

Biotin Anti-Mouse CD90 Antibody [M5/49.4.1]

AGEL3162
Assay Genie
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityMouse
TargetThy1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Biotin Anti-Mouse CD90 Antibody [M5/49.4.1]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry
  • Applications Supplier
    FCM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    M5/49.4.1
  • Conjugate
    Biotin
  • Gene ID21838
  • Target name
    Thy1
  • Target description
    thymus cell antigen 1, theta
  • Target synonyms
    CD90, T25, Thy-1, Thy-1.2, Thy1.1, Thy1.2, thy-1 membrane glycoprotein, thy-1 antigen
  • Host
    Rat
  • Protein IDP01831
  • Protein Name
    Thy-1 membrane glycoprotein
  • Scientific Description
    Biotin Anti-Mouse CD90 Antibody [M5/49.4.1] is a high-performance monoclonal that specifically targets Mouse CD90, delivering exceptional specificity and reliability for your research applications. Rigorously validated for FCM, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Mouse samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microg format to meet your experimental needs. For optimal performance, store at 4°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rat
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of CD90/Thy1 using anti-CD90/Thy1 antibody (A01818). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rai thymus tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD90/Thy1 antigen affinity purified polyclonal antibody (Catalog # A01818) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD90/Thy1 at approximately 22 kDa. The expected band size for CD90/Thy1 is at 18 kDa.
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