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ProductsBack/Rabbit anti-rat Thy-1/CD90

Rabbit anti-rat Thy-1/CD90

MUB2058
Nordic-MUbio
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
0.1 ml
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Rabbit anti-rat Thy-1/CD90
  • Delivery Days Customer
    7
  • Application Supplier Note
    Thy-1 is suitable for the detection of rat and mouse Thy-1 by immunohistochemical staining of frozen tissue sections. After drying, the sections can be either left unfixed or fixed with acetone at 4°C for 4 min or 95% ethanol at 4°C for 15 min or 4% formaldehyde in phosphate buffered saline pH 7.4 at 20°C for 15 min. Also isolated cells can be immunocytochemically stained by this antiserum. Optimal antibody dilutions for the different applications should be determined by titration. Recommended range 1:50 - 1:100.
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Applications Supplier
    Immunocytochemistry;Immunohistochemistry (frozen)
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Host
    Rabbit
  • Source
    Thy-1 antibody is a rabbit polyclonal antiserum obtained from rabbits immunised with purified Thy-1 derived from rat brain.
  • Reactivity Supplier
    Rat;Mouse
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD90/Thy1 using anti-CD90/Thy1 antibody (A01818). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rai thymus tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD90/Thy1 antigen affinity purified polyclonal antibody (Catalog # A01818) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD90/Thy1 at approximately 22 kDa. The expected band size for CD90/Thy1 is at 18 kDa.
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