Bio-Connect

Anti-TIGIT [4D4]

Ab00973-1.69
Absolute Antibody
ApplicationsFlow Cytometry, Other Application
Product group Antibodies
ReactivityMouse
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-TIGIT [4D4]
  • Delivery Days Customer
    7
  • Antibody Specificity
    4D4 specifically recognises TIGIT.
  • Application Supplier Note
    4D4 is an agonistic anti-TIGIT antibody, and addition of plate bound 4D4 to anti-CD3/anti-CD28 stimulated T cells was shown to inhibit their proliferation (Joller 2011). Further, 4D4 has been used in order to identify genes that are downstream of TIGIT signaling in Tregs, as TIGIT engagement might modulate the Treg phenotype in tumor tissue (Liu 2016).
  • Applications
    Flow Cytometry, Other Application
  • Applications Supplier
    Agonist; Fc
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4D4
  • Host
    Mouse
  • Protein IDP86176
  • Protein Name
    T-cell immunoreceptor with Ig and ITIM domains
  • Scientific Description
    This is a bispecific anti-mouse antibody created by fusing anti-PDL-1 VHH domains to the C-terminus of the heavy chains) of the anti-mouse TIGIT antibody clone 4D4 in a mouse IgG1 D265 format. The mouse IgG1 D265 a format is an Fc-silenced format, preventing ADCC and CDC.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Reactivity Supplier Note
    Armenian hamsters were immunized with recombinant mouse TIGIT tetramers by a combination of s.c. and food pad immunization and booster injections. Draining lymph nodes were fused with Sp2/0-Ag14 cells, selected in HAT (hypoxanthine/aminopterin/thymidine) medium and supernatants were screened for specificity by ELISA and flow cytometry using TIGIT-transfectants.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203