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Anti-TIM-3 [8E11]

AB03432-10.0-BT
Absolute Antibody
ApplicationsFlow Cytometry, ELISA
Product group Antibodies
ReactivityHuman
TargetHAVCR2
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-TIM-3 [8E11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody can be used in the detection of human TIM-3 using ELISA. The binding characterization of this antibody for human TIM-3 was done using indirect immunofluorescence labelling and flow cytometry (CN106632675).
  • Applications
    Flow Cytometry, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8E11
  • Gene ID84868
  • Target name
    HAVCR2
  • Target description
    hepatitis A virus cellular receptor 2
  • Target synonyms
    CD366, HAVcr-2, KIM-3, SPTCL, TIM3, TIMD-3, TIMD3, Tim-3, hepatitis A virus cellular receptor 2, T cell immunoglobulin mucin 3, T-cell immunoglobulin and mucin domain 3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin family member 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, kidney injury molecule-3
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDQ8TDQ0
  • Protein Name
    Hepatitis A virus cellular receptor 2
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TIM 3 using anti-TIM 3 antibody (A00657-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 3 antigen affinity purified polyclonal antibody (Catalog # A00657-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 3 at approximately 45-70KD. The expected band size for TIM 3 is at 33KD.
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