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Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Anti-TIMM8A Antibody

HPA003628
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTIMM8A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TIMM8A
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1678
  • Target name
    TIMM8A
  • Target description
    translocase of inner mitochondrial membrane 8A
  • Target synonyms
    DDP, DDP1, DFN1, MTS, TIM8, mitochondrial import inner membrane translocase subunit Tim8 A, X-linked deafness dystonia protein, deafness dystonia protein 1, deafness/dystonia peptide, translocase of inner mitochondrial membrane 8 homolog A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO60220
  • Protein Name
    Mitochondrial import inner membrane translocase subunit Tim8 A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TIMM8A/DDP using anti-TIMM8A/DDP antibody (A07659-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HEPG2 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: monkey kidney tissue lysates, Lane 8: rat brain tissue lysates, Lane 9: rat liver tissue lysates, Lane 10: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMM8A/DDP antigen affinity purified polyclonal antibody (Catalog # A07659-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMM8A/DDP at approximately 13-14KD. The expected band size for TIMM8A/DDP is at 11KD.
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