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TIMM8A Antibody

LS-C830542
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTIMM8A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TIMM8A Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:40 - 1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID1678
  • Target name
    TIMM8A
  • Target description
    translocase of inner mitochondrial membrane 8A
  • Target synonyms
    DDP; DDP1; deafness dystonia protein 1; deafness/dystonia peptide; DFN1; mitochondrial import inner membrane translocase subunit Tim8 A; MTS; TIM8; translocase of inner mitochondrial membrane 8 homolog A; X-linked deafness dystonia protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TIMM8A/DDP using anti-TIMM8A/DDP antibody (A07659-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HEPG2 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: monkey kidney tissue lysates, Lane 8: rat brain tissue lysates, Lane 9: rat liver tissue lysates, Lane 10: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIMM8A/DDP antigen affinity purified polyclonal antibody (Catalog # A07659-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIMM8A/DDP at approximately 13-14KD. The expected band size for TIMM8A/DDP is at 11KD.
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