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Anti-TLR7 Antibody

HY-P990520
MedChem Express
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Anti-TLR7 Antibody
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Estimated Purity
    99.0
  • Scientific Description
    Anti-TLR7 Antibody is a CHO-expressed human antibody that targets TLR7. Anti-TLR7 Antibody contains huIgG1 heavy chain and hukappa light chain, with a predicted molecular weight (MW) of 145 kDa. The isotype control for Anti-TLR7 Antibody can refer to Human IgG1 kappa, Isotype Control (HY-P99001).
  • Storage Instruction
    -80°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of TLR7 using anti-TLR7 antibody (PB9452). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate Lane 2: COLO320 Whole Cell Lysate Lane 3: JURKAT Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR7 antigen affinity purified polyclonal antibody (Catalog # PB9452) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR7 at approximately 121KD. The expected band size for TLR7 is at 84KD.
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