Figure 1. Western blot analysis of TLR7 using anti-TLR7 antibody (PB9452). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate Lane 2: COLO320 Whole Cell Lysate Lane 3: JURKAT Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR7 antigen affinity purified polyclonal antibody (Catalog # PB9452) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR7 at approximately 121KD. The expected band size for TLR7 is at 84KD.
Figure 1. Western blot analysis of TLR7 using anti-TLR7 antibody (PB9452). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate Lane 2: COLO320 Whole Cell Lysate Lane 3: JURKAT Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TLR7 antigen affinity purified polyclonal antibody (Catalog # PB9452) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TLR7 at approximately 121KD. The expected band size for TLR7 is at 84KD.
Anti-TLR7 Antibody Picoband(r)
PB9452-DYLIGHT550
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Human, Mouse, Rat
TargetTLR7
Overview
- SupplierBoster Bio
- Product NameAnti-TLR7 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for TLR7 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateDyLight 550
- Gene ID51284
- Target nameTLR7
- Target descriptiontoll like receptor 7
- Target synonymsIMD74; TLR7-like; toll-like receptor 7; toll-like receptor 7-like
- HostRabbit
- IsotypeIgG
- Protein IDQ9NYK1
- Protein NameToll-like receptor 7
- Scientific DescriptionBoster Bio Anti-TLR7 Antibody Picoband® catalog # PB9452. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityBovine, Human, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203