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Immunofluorescence analysis of HeLa cells, using TM16A Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using TM16A Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using TM16A Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-TM16A ANO1 Antibody

A30508
Boster Bio
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetANO1
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-TM16A ANO1 Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Gene ID55107
  • Target name
    ANO1
  • Target description
    anoctamin 1
  • Target synonyms
    DOG1, INDMS, MYMY7, ORAOV2, TAOS2, TMEM16A, anoctamin-1, Ca2+-activated Cl- channel, anoctamin 1, calcium activated chloride channel, calcium activated chloride channel, discovered on gastrointestinal stromal tumors protein 1, oral cancer overexpressed 2, transmembrane protein 16A (eight membrane-spanning domains), tumor-amplified and overexpressed sequence 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5XXA6
  • Protein Name
    Anoctamin-1
  • Scientific Description
    Boster Bio Anti-TM16A ANO1 Antibody (Catalog# A30508). Tested in ICC, IF, ELISA applications. This antibody reacts with Human, Mouse.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TMEM16A using anti-TMEM16A antibody (A00713). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human HepG2 whole cell lysate, Lane 3: human A549 whole cell lysate, Lane 4: human PANC-1 whole cell lysate, Lane 5: human SK-OV-3 whole cell lysate, Lane 6: human SGC-7901 whole cell lysate, Lane 7: human COLO-320 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMEM16A antigen affinity purified polyclonal antibody (Catalog # A00713) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMEM16A at approximately 130KD. The expected band size for TMEM16A is at 114KD.
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