Bio-Connect
Anti-TNFSF10 Antibody

Anti-TNFSF10 Antibody

A100072
Antibodies.com
ApplicationsWestern Blot, ELISA
Product group Antibodies
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-TNFSF10 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to TNFSF10.
  • UNSPSC
    12352203

Related products

Product group Antibodies
Assay Genie
TargetTNFSF10
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetTNFSF10
  • SizePrice
Lane 1: Mouse lung lysates; Lane 2: U251 cell lysates probed with Anti–TRAIL Polyclonal Antibody, Unconjugated (bs-1214R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetTNFSF10
  • SizePrice
Product group Antibodies
References
GeneTex
ApplicationsWestern Blot
TargetTNFSF10
  • SizePrice
Immunohistochemical staining of human lung shows moderate membranous and cytoplasmic positivity in macrophages and pneumocytes.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
TargetTNFSF10
  • SizePrice
Western Blot analysis of HuvEc cells using TRAIL Polyclonal Antibody
Product group Antibodies
Cusabio
TNFSF10 AntibodyCSB-PA004326
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetTNFSF10
  • SizePrice
Product group Antibodies
Biosynth
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetTNFSF10
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsELISA
TargetTNFSF10
  • SizePrice
Figure 1. Western blot analysis of TRAIL using anti-TRAIL antibody (A00466-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human THP-1 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAIL antigen affinity purified polyclonal antibody (Catalog # A00466-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAIL at approximately 35KD. The expected band size for TRAIL is at 33KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetTNFSF10
  • SizePrice