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Immunohistochemical staining of human lung shows moderate membranous and cytoplasmic positivity in macrophages and pneumocytes.
Immunohistochemical staining of human lung shows moderate membranous and cytoplasmic positivity in macrophages and pneumocytes.
Immunohistochemical staining of human lung shows moderate membranous and cytoplasmic positivity in macrophages and pneumocytes.

Anti-TNFSF10 Antibody

HPA054938
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTNFSF10
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TNFSF10
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8743
  • Target name
    TNFSF10
  • Target description
    TNF superfamily member 10
  • Target synonyms
    APO2L, Apo-2L, CD253, TANCR, TL2, TNLG6A, TRAIL, tumor necrosis factor ligand superfamily member 10, Apo-2 ligand, TNF-related apoptosis inducing ligand TRAIL, chemokine tumor necrosis factor ligand superfamily member 10, tumor necrosis factor (ligand) family, member 10, tumor necrosis factor (ligand) superfamily, member 10, tumor necrosis factor ligand 6A, tumor necrosis factor superfamily member 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50591
  • Protein Name
    Tumor necrosis factor ligand superfamily member 10
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of TRAIL using anti-TRAIL antibody (A00466-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human THP-1 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAIL antigen affinity purified polyclonal antibody (Catalog # A00466-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAIL at approximately 35KD. The expected band size for TRAIL is at 33KD.
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