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Western Blot analysis of HuvEc cells using TRAIL Polyclonal Antibody
Western Blot analysis of HuvEc cells using TRAIL Polyclonal Antibody
Western Blot analysis of HuvEc cells using TRAIL Polyclonal Antibody

TNFSF10 Antibody

CSB-PA004326
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTNFSF10
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Overview

  • Supplier
    Cusabio
  • Product Name
    TNFSF10 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8743
  • Target name
    TNFSF10
  • Target description
    TNF superfamily member 10
  • Target synonyms
    Apo-2 ligand; APO2L; Apo-2L; CD253; chemokine tumor necrosis factor ligand superfamily member 10; TL2; TNF-related apoptosis inducing ligand TRAIL; TNLG6A; TRAIL; tumor necrosis factor (ligand) family, member 10; tumor necrosis factor (ligand) superfamily, member 10; tumor necrosis factor apoptosis-inducing ligand splice variant delta; tumor necrosis factor ligand 6A; tumor necrosis factor ligand superfamily member 10; tumor necrosis factor superfamily member 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50591
  • Protein Name
    Tumor necrosis factor ligand superfamily member 10
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Lane 1: Mouse lung lysates; Lane 2: U251 cell lysates probed with Anti–TRAIL Polyclonal Antibody, Unconjugated (bs-1214R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.
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Immunohistochemical staining of human lung shows moderate membranous and cytoplasmic positivity in macrophages and pneumocytes.
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Figure 1. Western blot analysis of TRAIL using anti-TRAIL antibody (A00466-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human THP-1 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAIL antigen affinity purified polyclonal antibody (Catalog # A00466-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAIL at approximately 35KD. The expected band size for TRAIL is at 33KD.
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