Figure 1. Western blot analysis of TNRC6C using anti-TNRC6C antibody (A09089-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNRC6C antigen affinity purified polyclonal antibody (Catalog # A09089-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNRC6C at approximately 210 kDa. The expected band size for TNRC6C is at 176 kDa.
Figure 1. Western blot analysis of TNRC6C using anti-TNRC6C antibody (A09089-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNRC6C antigen affinity purified polyclonal antibody (Catalog # A09089-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNRC6C at approximately 210 kDa. The expected band size for TNRC6C is at 176 kDa.
Anti-TNRC6C Antibody Picoband(r)
A09089-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetTNRC6C
Overview
- SupplierBoster Bio
- Product NameAnti-TNRC6C Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID57690
- Target nameTNRC6C
- Target descriptiontrinucleotide repeat containing adaptor 6C
- Target synonymstrinucleotide repeat containing 6C; trinucleotide repeat-containing gene 6C protein
- HostRabbit
- Protein IDQ9HCJ0
- Protein NameTrinucleotide repeat-containing gene 6C protein
- Scientific DescriptionBoster Bio Anti-TNRC6C Antibody Picoband® catalog # A09089-1. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203