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Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of non germinal center cells.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of non germinal center cells.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of non germinal center cells.

Anti-TNRC6C Antibody

HPA022007
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTNRC6C
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TNRC6C Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57690
  • Target name
    TNRC6C
  • Target description
    trinucleotide repeat containing adaptor 6C
  • Target synonyms
    trinucleotide repeat-containing gene 6C protein, trinucleotide repeat containing 6C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HCJ0
  • Protein Name
    Trinucleotide repeat-containing gene 6C protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TNRC6C using anti-TNRC6C antibody (A09089-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNRC6C antigen affinity purified polyclonal antibody (Catalog # A09089-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNRC6C at approximately 210 kDa. The expected band size for TNRC6C is at 176 kDa.
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