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Western Blot Positive WB detected in: Hela whole cell lysate All lanes: TNRC6C antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 176, 180 kDa Observed band size: 176 kDa
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: TNRC6C antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 176, 180 kDa Observed band size: 176 kDa
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: TNRC6C antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 176, 180 kDa Observed band size: 176 kDa

TNRC6C Antibody

CSB-PA862070LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTNRC6C
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Overview

  • Supplier
    Cusabio
  • Product Name
    TNRC6C Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57690
  • Target name
    TNRC6C
  • Target description
    trinucleotide repeat containing adaptor 6C
  • Target synonyms
    trinucleotide repeat-containing gene 6C protein, trinucleotide repeat containing 6C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HCJ0
  • Protein Name
    Trinucleotide repeat-containing gene 6C protein
  • Scientific Description
    Plays a role in RNA-mediated gene silencing by micro-RNAs (miRNAs). Required for miRNA-dependent translational repression of complementary mRNAs by argonaute family proteins. As scaffoldng protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TNRC6C using anti-TNRC6C antibody (A09089-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNRC6C antigen affinity purified polyclonal antibody (Catalog # A09089-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNRC6C at approximately 210 kDa. The expected band size for TNRC6C is at 176 kDa.
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